[Research of cytoskeleton-dependent mechanisms of contractile activity regulation in the smooth muscles].

ABSTRACT

Influence of modulation of cytoskeleton by colchicine, vinblastine and cytochalasine B on contractile reactions of smooth muscles has been investigated by mechanographical method, by the methods of the double sucrose gup junction. Ratio F/G-actin in smooth muscle cells defined a method of a fluorescent microscopy. Microfilaments in a greater degree than microtubule are involved in regulation of reductions caused by hyperpotassic-induced reductions of membrane of smooth muscle segments of the rat aorta and generation of action potentials and reductions smooth muscle cells from guinea pig urethra. Reductions of vascular segments of aorta in rats caused by a hyperosmotic solution depend on condition of microfilaments and microtubules, whereas reductions in isoosmotic striction cells depend on condition of microfilaments. The last are involved in mechanisms of phenylephrine influence on mechanical strain of vascular segments of the rat aorta. Contrary to that, microtubules are involved in stimulation by phenylephrine electric and contractile activity the smooth muscle cells guinea pig urethra. Oppressiof contractile activity of smooth muscle segments of the rat aorta is cAMP-mediated and depends on condition of microfilaments of cytoskeleton, while action potentials and reductions smooth muscle cells of a ureter depend on condition of microtubules.