[Isolation of isoforms and crystallization of endonucleases of Serratia marcescens]. / Razdelenie izoform i kristallizatsiia éndonukleazy Serratia marcescens.
Bioorg Khim
; 16(12): 1678-82, 1990 Dec.
Article
em Ru
| MEDLINE
| ID: mdl-1965284
ABSTRACT
Two isoforms of an extracellular endonuclease, nuclease Sm1 and nuclease Sm2, were isolated from the culture filtrate of Serratia marcescens strain B10 M1 by the ligand-exchange chromatography on iminodiacetate-agarose in Cu2(+)-form, and chromatography on phosphocellulose and DEAE-Toyopearl 650S. The pI for nucleases Sm1 and Sm2 were found to be 7.1 and 6.7, respectively. The amino acid analysis and N-terminal amino acid sequencing of the proteins showed a significant degree of homology between the enzymes. The secondary structure of nuclease Sm2 was calculated. Crystals of nuclease Sm2 were obtained with the space group P2(1)2(1)2(1), a 69.0; b 106.7; c 74.8 A.
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Serratia marcescens
/
Endodesoxirribonucleases
/
Endonucleases
/
Endorribonucleases
/
Isoenzimas
Idioma:
Ru
Revista:
Bioorg Khim
Ano de publicação:
1990
Tipo de documento:
Article