C3PO, an endoribonuclease that promotes RNAi by facilitating RISC activation.
Science
; 325(5941): 750-3, 2009 Aug 07.
Article
em En
| MEDLINE
| ID: mdl-19661431
ABSTRACT
The catalytic engine of RNA interference (RNAi) is the RNA-induced silencing complex (RISC), wherein the endoribonuclease Argonaute and single-stranded small interfering RNA (siRNA) direct target mRNA cleavage. We reconstituted long double-stranded RNA- and duplex siRNA-initiated RISC activities with the use of recombinant Drosophila Dicer-2, R2D2, and Ago2 proteins. We used this core reconstitution system to purify an RNAi regulator that we term C3PO (component 3 promoter of RISC), a complex of Translin and Trax. C3PO is a Mg2+-dependent endoribonuclease that promotes RISC activation by removing siRNA passenger strand cleavage products. These studies establish an in vitro RNAi reconstitution system and identify C3PO as a key activator of the core RNAi machinery.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Transporte
/
Proteínas de Drosophila
/
Complexo de Inativação Induzido por RNA
/
Interferência de RNA
/
Drosophila melanogaster
Limite:
Animals
Idioma:
En
Revista:
Science
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Estados Unidos