Probing key targets in insulin signaling and adipogenesis using a methanolic extract of Costus pictus and its bioactive molecule, methyl tetracosanoate.
Biotechnol Lett
; 31(12): 1837-41, 2009 Dec.
Article
em En
| MEDLINE
| ID: mdl-19693444
ABSTRACT
A methanolic extract of Costus pictus (CPME) showed optimum anti-diabetic activity at 100 ng/ml. Bioactivity-guided purification of CPME led to the isolation of methyl tetracosanoate (MT) which showed an optimum glucose uptake at 1 ng/ml. CPME at 10 mug/ml inhibited adipogenesis whereas fully differentiated adipocytes exhibited a 3-fold increase in lipid accumulation compared to pre-adipocytes. Gene and protein expression of key targets in insulin signaling and adipogenesis pathway revealed that CPME exhibited anti-diabetic activity along with anti-adipogenic activity whereas MT demonstrated only anti-diabetic activity.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Extratos Vegetais
/
Transdução de Sinais
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Costus
/
Adipogenia
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Ácidos Graxos
/
Hipoglicemiantes
Limite:
Animals
Idioma:
En
Revista:
Biotechnol Lett
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Índia