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Fission yeast IQGAP arranges actin filaments into the cytokinetic contractile ring.
Takaine, Masak; Numata, Osamu; Nakano, Kentaro.
Afiliação
  • Takaine M; Department of Structural Biosciences, Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan. masaktakaine@gmail.com
EMBO J ; 28(20): 3117-31, 2009 Oct 21.
Article em En | MEDLINE | ID: mdl-19713940
The contractile ring (CR) consists of bundled actin filaments and myosin II; however, the actin-bundling factor remains elusive. We show that the fission yeast Schizosaccharomyces pombe IQGAP Rng2 is involved in the generation of CR F-actin and required for its arrangement into a ring. An N-terminal fragment of Rng2 is necessary for the function of Rng2 and is localized to CR F-actin. In vitro the fragment promotes actin polymerization and forms linear arrays of F-actin, which are resistant to the depolymerization induced by the actin-depolymerizing factor Adf1. Our findings indicate that Rng2 is involved in the generation of CR F-actin and simultaneously bundles the filaments and regulates its dynamics by counteracting the effects of Adf1, thus enabling the reconstruction of CR F-actin bundles, which provides an insight into the physical properties of the building blocks that comprise the CR.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Schizosaccharomyces / Citoesqueleto de Actina / Proteínas de Ciclo Celular / Proteínas Ativadoras de GTPase / Proteínas de Schizosaccharomyces pombe / Citocinese Limite: Animals Idioma: En Revista: EMBO J Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Japão País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Schizosaccharomyces / Citoesqueleto de Actina / Proteínas de Ciclo Celular / Proteínas Ativadoras de GTPase / Proteínas de Schizosaccharomyces pombe / Citocinese Limite: Animals Idioma: En Revista: EMBO J Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Japão País de publicação: Reino Unido