Parthenogenesis and somatic cell nuclear transfer in sheep oocytes using Polscope.
Indian J Exp Biol
; 47(7): 550-8, 2009 Jul.
Article
em En
| MEDLINE
| ID: mdl-19761038
ABSTRACT
Parthenogenesis and Somatic cell nuclear transfer (SCNT) techniques, offer a unique approach to manipulate the genetic composition of derived human embryonic stem cells - an essential step if the full opportunities for disease modeling, drug discovery or individualized stem cell therapy are to be realized. The present study describes the use of sheep oocytes to acquire expertise and establish methods to reconstruct embryos for obtaining blastocysts before venturing into human SCNT where the oocytes are a very precious starting material. Maturation of sheep eggs in vitro for 20-24 hr resulted in 65% metaphase II (MII) eggs which were either parthenogenetically activated using calcium ionomycin or ethanol or subjected to SCNT using cumulus cell as somatic cell. Sixteen blastocysts were produced by parthenogenetic activation of 350 eggs whereas reconstructed embryos, after SCNT carried out in 139 eggs, progressed only up to morula stage. The procedure of parthenogenesis and SCNT will be useful to generate autologous ES cells using human eggs.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oócitos
/
Partenogênese
/
Técnicas de Transferência Nuclear
Limite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
Indian J Exp Biol
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Índia
País de publicação:
IN
/
INDIA
/
ÍNDIA