N255 is a key residue for recognition by a monoclonal antibody which protects against Yersinia pestis infection.
Vaccine
; 27(50): 7073-9, 2009 Nov 23.
Article
em En
| MEDLINE
| ID: mdl-19786138
ABSTRACT
Mab7.3 to Yersinia pestis LcrV antigen (LcrV(Ype)) protected J774A.1 macrophages in vitro from killing by a Yersinia pseudotuberculosis strain expressing LcrV(Ype). Of 4 site-directed mutations in the coiled-coil region (148-169) and 7 mutations in the 225-255 sequence of LcrV(Ype), only the mutation of N255 to D255, abrogated the binding of Mab7.3 and reduced its protective capacity against plague. Since the Mab7.3 epitope in LcrV(Ype) (135-275) encompasses a region (136-180) thought to be exposed on the injectisome, we suggest that Mab7.3 protects by binding to LcrV(Ype) and interfering with protein-protein interactions necessary for type three secretion.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peste
/
Proteínas Citotóxicas Formadoras de Poros
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Anticorpos Antibacterianos
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Anticorpos Monoclonais
/
Antígenos de Bactérias
Limite:
Animals
Idioma:
En
Revista:
Vaccine
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Reino Unido