Oriented immobilization of antibody fragments on Ni-decorated single-walled carbon nanotube devices.
ACS Nano
; 3(11): 3649-55, 2009 Nov 24.
Article
em En
| MEDLINE
| ID: mdl-19795840
ABSTRACT
We herein demonstrate that Ni-decorated single-walled carbon nanotube field effect transistors (SWNT-FETs) combined with antibody fragments can be used as effective biosensing platforms. Nanoscales Ni particles 20 to 60 nm in diameter were formed on the sidewalls of SWNT-FETs using an electrochemical method. Carcinoembryonic antigen (CEA)-binding single chain variable fragments (scFvs) with a hexahistidine tag [(his)(6)] were synthesized using genetic engineering, and ordered immobilization of anti-CEA ScFvs on Ni nanoparticles was achieved by exploiting the specific interaction between hexahistidine and Ni. Whereas randomly oriented anti-CEA scFvs did not impart a noticeable change of conductance upon addition of CEA, a clear increase in conductance was observed using Ni-decorated SWNT-FETs functionalized with engineered scFvs.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Nanotubos de Carbono
/
Eletroquímica
/
Anticorpos Imobilizados
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Anticorpos de Cadeia Única
/
Níquel
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
ACS Nano
Ano de publicação:
2009
Tipo de documento:
Article