[Preparation and characterization of monoclonal antibodies to rabies virus CVS-11 strain].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
; 23(1): 68-70, 2009 Feb.
Article
em Zh
| MEDLINE
| ID: mdl-19799024
ABSTRACT
OBJECTIVE:
To get the high specific and sensitive mononclonal antibodies against RV.METHODS:
The myeloma cell line SP2/0 fused with the spleen cell of 6 - 8 weeks old BALB/c mice immunized with the CVS-11 virus antigen. The hybridized fusing cells were chosen by indirect ELISA detection and the positive hybridizing cells were amplified through mouse abdomen injection and the mouse McAbs ascites was purified by Protein A Sepharose 4 Fast Flow (Pharmacia Company). The specificity and sensitivity of the McAbs was identified by indirect ELISA and indirect DFA test.RESULTS:
The cell fusion rate reachs 100% and the indirect ELISA results showed that the McAbs ascites titer were 1 x 10(4), 1 x 10(5), 1 x 10(4) and 1 x 10(5); The immunoglobulin G type McAbs show no cross reaction with other related viruses.CONCLUSION:
The high specific and sensitive mononclonal antibodies of RV can be used for rapid RV diagnosis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Raiva
/
Vírus da Raiva
/
Ensaio de Imunoadsorção Enzimática
/
Anticorpos Monoclonais
/
Anticorpos Antivirais
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
/
Female
/
Humans
Idioma:
Zh
Revista:
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
Assunto da revista:
VIROLOGIA
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
China