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A mycobacterial cyclic AMP phosphodiesterase that moonlights as a modifier of cell wall permeability.
Podobnik, Marjetka; Tyagi, Richa; Matange, Nishad; Dermol, Urska; Gupta, Arun K; Mattoo, Rohini; Seshadri, Kothandaraman; Visweswariah, Sandhya S.
Afiliação
  • Podobnik M; Laboratory for Biosynthesis and Biotransformation, National Institute of Chemistry of Slovenia, Hajdrihova 19,1000 Ljubljana, Slovenia. marjetka.podobnik@ki.si
J Biol Chem ; 284(47): 32846-57, 2009 Nov 20.
Article em En | MEDLINE | ID: mdl-19801656
Mycobacterium tuberculosis utilizes many mechanisms to establish itself within the macrophage, and bacterially derived cAMP is important in modulating the host cellular response. Although the genome of M. tuberculosis is endowed with a number of mammalian-like adenylyl cyclases, only a single cAMP phosphodiesterase has been identified that can decrease levels of cAMP produced by the bacterium. We present the crystal structure of the full-length and sole cAMP phosphodiesterase, Rv0805, found in M. tuberculosis, whose orthologs are present only in the genomes of slow growing and pathogenic mycobacteria. The dimeric core catalytic domain of Rv0805 adopts a metallophosphoesterase-fold, and the C-terminal region builds the active site and contributes to multiple substrate utilization. Localization of Rv0805 to the cell wall is dependent on its C terminus, and expression of either wild type or mutationally inactivated Rv0805 in M. smegmatis alters cell permeability to hydrophobic cytotoxic compounds. Rv0805 may therefore play a key role in the pathogenicity of mycobacteria, not only by hydrolyzing bacterial cAMP, but also by moonlighting as a protein that can alter cell wall functioning.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Parede Celular / 3',5'-AMP Cíclico Fosfodiesterases / Mycobacterium tuberculosis Idioma: En Revista: J Biol Chem Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Eslovênia País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Parede Celular / 3',5'-AMP Cíclico Fosfodiesterases / Mycobacterium tuberculosis Idioma: En Revista: J Biol Chem Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Eslovênia País de publicação: Estados Unidos