Denaturation resistance of beta-lactoglobulin in monomolecular films at the air-water interface.
J Phys Chem B
; 113(43): 14513-20, 2009 Oct 29.
Article
em En
| MEDLINE
| ID: mdl-19810743
ABSTRACT
Using X-ray reflectometry we report strong differences in the denaturation response of beta-lactoglobulin adsorbed as a monomolecular film at the air-water interface from that observed in mixed denaturant/beta-lactoglobulin bulk solutions. Using the "flow trough" technique an isolated monomolecular film of the protein showed little change in structure when subjected to a 4.0 M guanidinium hydrochloride substrate. Unlike the bulk solution where a new protein layer structure appears, small changes in the protein packing and the roughness of the film are the only evidence of change. These parameters have been studied as a function of denaturant concentration and film quality. The strength of the response depends on the degree of perfection of the originally formed film; quickly formed films are more easily denatured. As the response is so subtle, possible interfering effects such as denaturant release of protein adsorbed on the trough have been quantified.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Água
/
Ar
/
Lactoglobulinas
Idioma:
En
Revista:
J Phys Chem B
Assunto da revista:
QUIMICA
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Austrália