Effect of 830 nm laser phototherapy on osteoblasts grown in vitro on Biosilicate scaffolds.
Photomed Laser Surg
; 28(1): 131-3, 2010 Feb.
Article
em En
| MEDLINE
| ID: mdl-19814702
ABSTRACT
OBJECTIVE:
The purpose of this study was (i) to develop a method for successfully seeding osteoblasts onto a glass-ceramic scaffold designed for use in clinical settings, and (ii) to determine whether the application of laser phototherapy at 830 nm would result in osteoblast proliferation on the glass-ceramic scaffold.BACKGROUND:
The use of bioscaffolds is considered a promising strategy for a number of clinical applications where tissue healing is sub-optimal. As in vitro osteoblast growth is a slow process, laser phototherapy could be used to stimulate osteoblast proliferation on bioscaffolds.METHODS:
A methodology was developed to seed an osteoblastic (MC3T3) cell line onto a novel glass-ceramic scaffold. Seeded scaffolds were irradiated with a single exposure of 830 nm laser at 10 J/cm(2) (at diode). Non-irradiated seeded scaffolds acted as negative controls. Cell proliferation was assessed seven days after irradiation.RESULTS:
Osteoblastic MC3T3 cells were successfully grown on discs composed of a glass-ceramic composite. Laser irradiation produced a 13% decrease in MC3T3 cell proliferation on glass-ceramic discs (mean +/- SD = 0.192 +/- 0.002) compared with control (non-irradiated) discs (mean +/-SD = 0.22 +/- 0.002).CONCLUSIONS:
Despite successful seeding of bioscaffolds with osteoblasts, laser phototherapy resulted in a reduction in cell growth compared to non-irradiated controls. Future research combining laser phototherapy and glass-ceramic scaffolds should take into account possible interactions of the laser with matrix compounds.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Osteoblastos
/
Cerâmica
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Terapia com Luz de Baixa Intensidade
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Proliferação de Células
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Alicerces Teciduais
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Lasers Semicondutores
Limite:
Animals
Idioma:
En
Revista:
Photomed Laser Surg
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Brasil