The Sfpi-1 proviral integration site of Friend erythroleukemia encodes the ets-related transcription factor Pu.1.
J Virol
; 65(1): 464-7, 1991 Jan.
Article
em En
| MEDLINE
| ID: mdl-1985210
ABSTRACT
Previous studies identified a common site (Sfpi-1) for proviral integration in immortalized Friend erythroleukemias. cDNAs corresponding to a 1.5-kb Sfpi-1 mRNA were isolated and sequenced. These were larger than an independently isolated Sfpi-1 cDNA described by researchers from another laboratory, and they contained common differences from that sequence, including in the coding region four extra nucleotides that altered the reading frame. The properly translated protein is identical to Pu.1, a transcription activation factor that is related to the ets oncogene family. Genetic methods were used to map Sfpi-1 with respect to other loci on mouse chromosome 2. Our results suggest that Pu.1 blocks erythroblast differentiation and thereby causes immortalization.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
/
Leucemia Experimental
/
Proteínas Oncogênicas de Retroviridae
/
Provírus
/
Proteínas de Ligação a DNA
/
Vírus da Leucemia Murina de Friend
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Virol
Ano de publicação:
1991
Tipo de documento:
Article