[Cilostazol induces metallothionein expression in vascular cells].
Yakugaku Zasshi
; 129(11): 1415-22, 2009 Nov.
Article
em Ja
| MEDLINE
| ID: mdl-19881214
ABSTRACT
Metallothionein (MT) is a cysteine-rich low molecular weight protein and thought to function in the detoxification of heavy metals and reactive oxygen species. We examined the induction of MT synthesis by cilostazol, an antiplatelet drug, in several vascular component cells to find a new pharmacological effect of cilostazol in vascular system. In human coronary artery endothelial cells, cilostazol significantly increased MT-IX and MT-IIA mRNA levels after the treatment for 6 h and endogenous MT-I/II protein levels after the treatment for 24 and 48 h. In addition, cadmium cytotoxicity was prevented by cilostazol in this endothelial cells. Moreover, cilostazol increased MT-IX and MT-IIA mRNA levels in human coronary artery smooth muscle cells, human brain microvascular endothelial cells, human brain microvascular pericytes and human colon carcinoma Caco-2 cells after the treatment for 6 h. Interestingly, cilostazol also increased MT-III mRNA level in brain microvascular endothelial cells more effectively than in other vascular cells. The present results suggest that cilostazol can protect the vascular system from toxic substances such as heavy metals via MT induction in vascular cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tetrazóis
/
Inibidores da Agregação Plaquetária
/
Células Endoteliais
/
Metalotioneína
/
Músculo Liso Vascular
Tipo de estudo:
Etiology_studies
Limite:
Humans
Idioma:
Ja
Revista:
Yakugaku Zasshi
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Japão