Investigation of porcine endogenous retrovirus in the conservation population of Ningxiang pig.
Transplant Proc
; 41(10): 4389-93, 2009 Dec.
Article
em En
| MEDLINE
| ID: mdl-20005405
ABSTRACT
Porcine endogenous retrovirus (PERV) varies between pig breeds. Screening and analysis of PERV in putative pig breeds may provide basic parameters to evaluate the biological safety of xenotransplantation from pigs to humans. In this study, PERV was investigated among the conservation population of the Ningxiang pig. The result revealed that the genotype of PERV distribution was subtype A, 100%; subtype B, 100%; and subtype C, 100%. The env sequences of PERV-A and -B showed 11 clones detected by KpnI and MboI digestion, indicating that there existed multiple variants of PERV-A and -B in the Ningxiang pig. Reverse transcriptase polymerase chain reaction results showed that PERV had transcriptional activity in these individuals. In addition, PERV A/C recombinant was detected in most individuals of Ningxiang pig. Because PERV A/C recombinants increase the potential infectious risk, the breed may not be a proper donor for xenotransplantation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Suínos
/
Retrovirus Endógenos
Limite:
Animals
/
Humans
País/Região como assunto:
Asia
Idioma:
En
Revista:
Transplant Proc
Ano de publicação:
2009
Tipo de documento:
Article