Interference RNA for in vivo Knock-down of gene expression or genome-wide screening using shRNA.
Methods Mol Biol
; 597: 189-209, 2010.
Article
em En
| MEDLINE
| ID: mdl-20013235
ABSTRACT
With the lack of tools available to manipulate the rat genome, alternative technologies have been investigated to generate loss-of-function rat models by gene invalidation. The recent demonstration that RNA interference (RNAi)-mediated gene silencing occurs in rodents has opened new opportunities for rat functional genetics. In this chapter, we provide some practical guidelines for RNAi working in rat, based on the recent design and development of mice and rat Knock down models.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Genômica
/
Interferência de RNA
/
Técnicas de Silenciamento de Genes
Tipo de estudo:
Diagnostic_studies
/
Guideline
/
Screening_studies
Limite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
França