A histopathological study of the pulp of dogs' teeth after induction of experimental pulp inflammation for different periods of time.

ABSTRACT

The purpose of this study was to investigate the reaction of the pulp of dogs' teeth after insertion of soft carious dentin from freshly extracted human teeth into the buccal cavities for short and longer periods of time. Forty-seven mature lower and upper teeth were used in this study. On the middle of the buccal side of the teeth, 3 x 5 mm class nu cavities were prepared, soft carious dentin from freshly extracted human teeth was inserted into the floor of the cavity, and those were filled with glass ionomer. The dogs were killed after 7, 14, 28 and 47 days using vital perfusion techniques. Six-micrometre sections were prepared and blindly evaluated by pathologist. An inflammatory reaction occurred in all of the samples. Moderate to severe inflammation were shown in all periods except in one in the 7-day period. Insertion of soft carious dentin gathered from freshly extracted human teeth into class nu cavities produced in dogs' teeth for 7 days is a reproducible process and could be used in study of pulp pathology.