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The spatial expression pattern of antimicrobial peptides across the healthy bovine udder.
Tetens, J; Friedrich, J J; Hartmann, A; Schwerin, M; Kalm, E; Thaller, G.
Afiliação
  • Tetens J; Institute of Animal Breeding and Husbandry, Christian-Albrechts-University Kiel, D-24098 Kiel, Germany. jtetens@tierzucht.uni-kiel.de
J Dairy Sci ; 93(2): 775-83, 2010 Feb.
Article em En | MEDLINE | ID: mdl-20105549
ABSTRACT
Antimicrobial peptides are key molecules in local host defense. With the aim to better understand the possible involvement of these peptides in the prevention of bovine mastitis, we determined, for the first time to our knowledge, the spatial pattern of constitutive expression of 5 bovine beta-defensins and bovine psoriasin (S100A7) across 5 localizations of the bovine mammary gland applying a quantitative real-time PCR approach. We observed 3 different expression patterns in the healthy udder 1) constitutive expression of the lingual and tracheal antimicrobial peptides (LAP and TAP), as well that of bovine neutrophil beta-defensins 4 and 10 (BNBD4 and BNBD10), is essentially restricted to the mammary lymph node; 2) bovine beta-defensin 1 (DEFB1) is mainly expressed in the cisternal epithelium and the Rosette of Fürstenberg; 3) strong constitutive mRNA expression of the calcium-binding protein S100A7, which is also known as psoriasin and which has been reported to be highly active against Escherichia coli, was detected in the streak canal. These results indicate a crucial role of S100A7 in the early-stage prevention of coliform mastitis, and the analyzed beta-defensins might be regarded as inducible weapons against already invading pathogens.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bovinos / Peptídeos Catiônicos Antimicrobianos / Glândulas Mamárias Animais Limite: Animals / Female / Humans Idioma: En Revista: J Dairy Sci Ano de publicação: 2010 Tipo de documento: Article País de afiliação: Alemanha

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bovinos / Peptídeos Catiônicos Antimicrobianos / Glândulas Mamárias Animais Limite: Animals / Female / Humans Idioma: En Revista: J Dairy Sci Ano de publicação: 2010 Tipo de documento: Article País de afiliação: Alemanha