Short communication: characterization of a monoclonal antibody for kappa-casein B of cow's milk.
J Dairy Sci
; 93(2): 796-800, 2010 Feb.
Article
em En
| MEDLINE
| ID: mdl-20105552
ABSTRACT
A monoclonal antibody (antik-B) against an oligopeptide of 23 AA corresponding to the region 131-153 of bovine kappa-casein (kappa-CN) B was generated using the Human Combinatorial Antibody Library (HuCAL) technology. Both AA substitutions distinguishing kappa-CN A and B are located in that region (positions 136 and 148). In this study, the reactivity of antik-B to milk samples collected from cows previously genotyped as CSN3*AA, CSN3*AB, and CSN3*BB was tested. According to Western blot results, antik-B recognized kappa-CN B and it showed no cross-reactivity toward kappa-CN A and other milk proteins. Furthermore, a modified Western blot method, urea-PAGE Western blot, was set up to assess the reactivity of antik-B toward all isoforms of kappa-CN B. In conclusion, antik-B was specific to kappa-CN B in milk and it seemed to be reactive toward all its isoforms.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Caseínas
/
Leite
/
Anticorpos Monoclonais
Limite:
Animals
Idioma:
En
Revista:
J Dairy Sci
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Itália