[Complete genome sequence analysis of Japanese encephalitis virus newly isolated in China].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
; 23(4): 245-7, 2009 Aug.
Article
em Zh
| MEDLINE
| ID: mdl-20108763
ABSTRACT
OBJECTIVE:
To study the complete genome sequence of Japanese encephalitis virus (JEV) strain XJ69 isolated in ZheJiang province and explore its evolution.METHODS:
Overlapping primers were designed according to the full-length genomes from GenBank. RT-PCR was used to amplify the fragments and RT-PCR products were cloned T vector, sequenced and analyzed.RESULTS:
The genome of strain XJ69 and XJP613 were 10 964 nucleotides in length with a single open reading frame encoding 3432 amino acids. Comparison of the complete genome sequences of different JEV isolates showed XJ69 and XJP613 were 83.5%-99.2% and 83.4%-99.4% nucleotide sequence homology among them respectively, which resulted in 94.8%-99.7% amino acid sequence homology. Phylogenetic analysis through PrM/C,E and full-length genome showed that the XJ69 and XJP613 strain belonged to genotype I.CONCLUSION:
The nucleotitede sequence and deduced amino acid sequence of XJ69 and XJP613 strain were similar to that of those of genotype I of Japanese encephalitis virus. It belonged to genotype I and were close to the isolates SH17M-07.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Genoma Viral
/
Encefalite Japonesa
/
Vírus da Encefalite Japonesa (Espécie)
Limite:
Animals
/
Humans
País/Região como assunto:
Asia
Idioma:
Zh
Revista:
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
Assunto da revista:
VIROLOGIA
Ano de publicação:
2009
Tipo de documento:
Article