Your browser doesn't support javascript.
loading
Tranilast stabilizes the accumulation and degranulation of resident mast cells while reducing cardiomyocyte apoptosis in a swine model of coronary microembolisation.
Zhang, Qing-Yong; Li, Jing-Bo; Wang, Zhi-Hua; Li, Xiao-Bo; Yin, Lian-Hua; Wei, Meng.
Afiliação
  • Zhang QY; Division of Cardiology, Number 6 People's Hospital, Jiaotong University, Shanghai 200233, China. yeakcardio@yahoo.com.cn
Clin Exp Pharmacol Physiol ; 37(5-6): 641-6, 2010 May.
Article em En | MEDLINE | ID: mdl-20132236
1. Coronary microembolisation (CME) is associated with progressive myocardial dysfunction, and mast cells (MC) might have an important role in myocardial apoptosis after CME. We investigated whether the MC stabilizer tranilast suppresses the accumulation and degranulation of MC while reducing cardiomyocyte apoptosis after CME. 2. We induced CME in miniswine by selective infusion of 15 x 10(4) microspheres (diameter 45 microm) into the left anterior descending artery. Some CME-induced miniswine were treated with the MC stabilizer tranilast (50 mg/kg, p.o., b.d.) beginning 2 weeks before CME, and thereafter throughout the experimental period; others received tranilast without CME; and sham-operated animals without CME served as controls. After 30 days, we assessed cardiomyocyte apoptosis by TUNEL assay and by the total number of MC and the number of degranulating MC using histology and transmission electron microscopy. The wall motion score index and left ventricular ejection fraction were studied by dobutamine stress echocardiography. 3. Coronary microembolisation was associated with increases in the total number of MC, the number of degranulating MC, and myocyte apoptosis. The number of total MC and degranulating MC and apoptotic cardiomyocytes over the anterior embolized myocardium after CME were significantly higher than those over the posterior control myocardium and anterior segments per animal without CME (P < 0.01). Tranilast administration to CME miniswine suppressed cardiomyocyte apoptosis while maintaining regional and global function, which was associated with reductions in the accumulation and degranulation of MC. 4. These findings suggest that tranilast suppresses the accumulation and degranulation of MC while reducing cardiomyocyte apoptosis after CME.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Degranulação Celular / Apoptose / Vasos Coronários / Miócitos Cardíacos / Embolia / Ortoaminobenzoatos / Mastócitos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Clin Exp Pharmacol Physiol Ano de publicação: 2010 Tipo de documento: Article País de afiliação: China País de publicação: Austrália

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Degranulação Celular / Apoptose / Vasos Coronários / Miócitos Cardíacos / Embolia / Ortoaminobenzoatos / Mastócitos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Clin Exp Pharmacol Physiol Ano de publicação: 2010 Tipo de documento: Article País de afiliação: China País de publicação: Austrália