Localization of cyclo-oxygenase-2 in human recurrent colorectal cancer.
Clin Invest Med
; 33(1): E22-9, 2010 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-20144265
AIM: The aim of this paper is to examine COX-2 expression in human recurrent colorectal carcinoma tissues using immunohistochemistry and quantative real-time PCR (qPCR). METHODS: Colon and rectal specimens were obtained from 26 patients with recurrent colorectal carcinomas. We examined COX-2 expression in human recurrent colorectal carcinoma tissues using immunohistochemistry and quantative real-time PCR (qPCR). RESULTS: In recurrent colorectal cancer a strong cytoplasmic and perinuclear staining of COX-2 was found. Moderate to strong immunosignals were detected in almost all of the carcinomas. We observed a strong specific staining of COX-2 in vascular endothelium. COX-2 immunoreactivity was also detected in stromal cells such as mononuclear cells, fibroblasts, and smooth muscle cells. The real-time PCR analyses demonstrated marked overexpression of the COX-2 gene in the cancer mucosa in concert with the immunohistochemistry data. CONCLUSION: We investigated COX-2 expression at the level of its protein as well as its messenger RNA in a series of recurrent colorectal cancers. These observations give additional information about the possibility that COX-2 could be involved in tumor promotion during colorectal cancer progression.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Colorretais
/
Ciclo-Oxigenase 2
/
Recidiva Local de Neoplasia
Limite:
Female
/
Humans
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Male
/
Middle aged
Idioma:
En
Revista:
Clin Invest Med
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Alemanha
País de publicação:
Canadá