Myocilin levels in primary open-angle glaucoma and pseudoexfoliation glaucoma human aqueous humor.
J Glaucoma
; 19(9): 569-75, 2010 Dec.
Article
em En
| MEDLINE
| ID: mdl-20179615
ABSTRACT
PURPOSE:
To determine the concentration of myocilin in primary open-angle glaucoma (POAG) and pseudoexfoliation glaucoma (PEXG) aqueous humor.METHODS:
Aqueous humor was collected during surgery from patients with POAG, PEXG, and elective cataract removal (control). Volume-equivalent aqueous samples were separated on sodium dodecyl sulfate-polyacrylamide gel electrophoresis gradient gels. Quantification of myocilin levels was performed using Western blots probed with 2 independent N-terminal polyclonal anti-myocilin antibodies (AB1 and AB2) followed by densitometry. Myocilin levels in aqueous humor were quantified by plotting the densitometry readings of the aqueous samples against a recombinant myocilin standard curve. Total protein concentration was determined by Bradford protein assay. Transforming growth factor ß 2 levels were assessed by enzyme-linked immunosorbent assay.RESULTS:
Myocilin levels are significantly elevated in human POAG aqueous humor when compared with control aqueous humor (AB1 0.66±0.53 ng/µL vs. 0.23±0.20 ng/µL, P<0.001; AB2 0.98±0.59 ng/µL vs. 0.65±0.5 ng/µL, P<0.03; mean±SD). Myocilin makes up a larger percent of the total protein in POAG aqueous humor compared with control aqueous (AB1 0.26±0.20% vs. 0.10±0.20%, P<0.001; AB2 0.43±0.32% vs. 0.28±0.18%, P<0.05). In contrast to POAG, myocilin levels were not elevated in PEXG aqueous humor when compared with control aqueous humor. No correlation between myocilin and transforming growth factor ß 2 levels was observed.CONCLUSIONS:
Myocilin is elevated in POAG, but not in PEXG aqueous humor.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Humor Aquoso
/
Glicoproteínas
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Glaucoma de Ângulo Aberto
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Síndrome de Exfoliação
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Proteínas do Citoesqueleto
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Proteínas do Olho
Limite:
Aged
/
Aged80
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Female
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Humans
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Male
/
Middle aged
Idioma:
En
Revista:
J Glaucoma
Assunto da revista:
OFTALMOLOGIA
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Estados Unidos