Biomaterials for high-throughput stem cell culture.
Curr Stem Cell Res Ther
; 5(3): 261-7, 2010 Sep.
Article
em En
| MEDLINE
| ID: mdl-20214555
ABSTRACT
A cell's microenvironment plays a primary role in defining cell fate during tissue development, physiological function, and pathological dysfunction. Understanding the key components and interactions within these microenvironments is critical for effective use of stem cells for disease modeling and therapeutic applications. Yet cell microenvironments are difficult to study, as there are tens or hundreds of parameters that can influence cell behavior simultaneously. Additionally, parameters such as cell-cell interactions, cell-ECM interactions, cell shape, soluble signals, and mechanical forces vary dynamically in 3-dimensional space and time. The number of relevant experimental conditions in these cell-based biological systems quickly becomes intractable using standard experimental platforms and techniques. A new set of strategies involving high-throughput experimental formats and 3-dimensional culture is required to achieve significant progress in understanding and exploiting stem cell biology. This mini-review describes bioengineering approaches that are enabling for high-throughput stem cell culture, screening and analysis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco
/
Materiais Biocompatíveis
/
Técnicas de Cultura de Células
Limite:
Humans
Idioma:
En
Revista:
Curr Stem Cell Res Ther
Assunto da revista:
TERAPEUTICA
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Estados Unidos