[Construction of eukaryotic expression vector of general type in reverse genetic systems of non-segmented negative strand RNA virus].
Bing Du Xue Bao
; 26(1): 65-70, 2010 Jan.
Article
em Zh
| MEDLINE
| ID: mdl-20329561
ABSTRACT
To construct eukaryotic expression vector of general type in reverse genetics systems of non-segmented negative strand RNA viruses, the multiple cloning site of the plasmid pVAX1 was replaced with HamRz cDNA sequence, a 9 sites linker and HdvRz cDNA sequence through the sequential addition of three adapters, the insertion of which could generate the correct 3' and 5' terminal sequences of the primary viral genomic RNA transcript and facilitate the assembly of the complete viral cDNA sequence. The sequences of the three adaptors were correct after identifying by restriction endonuclease digestions and sequencing. The constructed eukaryotic expression vector could not only be used to assemble viral genome, but also provide the basis for establishing the reverse genetic system rapidly.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírus de RNA
/
Eucariotos
/
Vetores Genéticos
Limite:
Animals
Idioma:
Zh
Revista:
Bing Du Xue Bao
Assunto da revista:
VIROLOGIA
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
China