Determination of lamotrigine in human serum by high-performance liquid chromatographytandem mass spectrometry.
Neurol Sci
; 31(6): 717-20, 2010 Dec.
Article
em En
| MEDLINE
| ID: mdl-20390433
ABSTRACT
A rapid and sensitive liquid chromatographytandem mass spectrometry method was developed and validated for quantification of lamotrigine in human serum. After a simple protein precipitation using acetonitrile, the analytes were separated on a Shideido 150 mm × 2.0 mm, 5 µm Capcell Pak C18 MG column using 70% acetonitrileas mobile phase at a flow rate of 200 µl/min. Lamotrigine was eluted at 1.98 min, ionized using electrospray ionization source, and then detected by multiple reaction monitoring mode. The precursor to product ion transitions of m/z 256.1109.0 was used to quantify. The analytical measurement range is from 0.1 to 20 µg/ml and the upper clinical reportable range is chosen to be 100 µg/ml. The method was successfully employed in a clinical application.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Triazinas
/
Espectrometria de Massas em Tandem
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Neurol Sci
Assunto da revista:
NEUROLOGIA
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Coréia do Sul