Morphological evaluation of cytoarchitectonics of aortic graft at the biotechnological stage with analysis of changes in laser-induced fluorescence spectra.
Bull Exp Biol Med
; 148(4): 684-8, 2009 Oct.
Article
em En
| MEDLINE
| ID: mdl-20396770
We performed a fluorescent microscopic examination of human and animal aortic grafts at different stages of decellularization. Treatment of aortic grafts with trypsin/EDTA solution for 48 h leads to their complete decellularization and preserved the connective tissue fiber backbone, which gains a netlike structure. The use of this protocol of decellularization leads to disappearance of subintimal calcium deposits in human aortic grafts. Differences in laser-induced fluorescence spectra of aortas before decellularization and at different stages of this process were revealed. Our findings suggest that the use of fluorescence induced by excimer lasers is promising for identification of the composition of biological tissues, analysis of their state, and the presence of changes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Aorta
/
Prótese Vascular
/
Transplantes
Tipo de estudo:
Guideline
/
Prognostic_studies
Limite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
Bull Exp Biol Med
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Federação Russa
País de publicação:
Estados Unidos