Construction of river model biofilm for assessing pesticide effects.
Arch Environ Contam Toxicol
; 60(1): 44-56, 2011 Jan.
Article
em En
| MEDLINE
| ID: mdl-20422166
ABSTRACT
Due to the high importance of biofilms on river ecosystems, assessment of pesticides' adverse effects is necessary but is impaired by high variability and poor reproducibility of both natural biofilms and those developed in the laboratory. We constructed a model biofilm to evaluate the effects of pesticides, consisting in cultured microbial strains, Pedobacter sp. 7-11, Aquaspirillum sp. T-5, Stenotrophomonas sp. 3-7, Achnanthes minutissima N71, Nitzschia palea N489, and/or Cyclotella meneghiniana N803. Microbial cell numbers, esterase activity, chlorophyll-a content, and the community structure of the model biofilm were examined and found to be useful as biological factors for evaluating the pesticide effects. The model biofilm was formed through the cooperative interaction of bacteria and diatoms, and a preliminary experiment using the herbicide atrazine, which inhibits diatom growth, indicated that the adverse effect on diatoms inhibited indirectly the bacterial growth and activity and, thus, the formation of the model biofilm. Toxicological tests using model biofilms could be useful for evaluating the pesticide effects and complementary to studies on actual river biofilms.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Atrazina
/
Bactérias
/
Poluentes Químicos da Água
/
Diatomáceas
/
Biofilmes
/
Herbicidas
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Arch Environ Contam Toxicol
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Japão