Glyceraldehyde-3-phosphate dehydrogenase interacts with phosphorylated Akt resulting from increased blood glucose in rat cardiac muscle.
FEBS Lett
; 584(13): 2796-800, 2010 Jul 02.
Article
em En
| MEDLINE
| ID: mdl-20488185
ABSTRACT
Here we describe the interaction of phosphorylated approximately 40 kDa protein with phosphorylated Akt which is a serine/threonine kinase resulting from increased blood glucose in rat cardiac muscle. Mass spectrometry analysis revealed that this protein was glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Furthermore, increase in Akt and GAPDH phosporylation and induction of their association were both observed after insulin stimulation in the H9c2 cell line derived from embryonic rat ventricle. Moreover, the activation of GAPDH was upregulated when the GAPDH phosphorylation was increased. Our data suggest that GAPDH phosphorylation and association with Akt by insulin treatment have some bearing on the enhancement of GAPDH activity.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Glicemia
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Proteínas Proto-Oncogênicas c-akt
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Gliceraldeído-3-Fosfato Desidrogenases
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Miocárdio
Limite:
Animals
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Japão