Spreading by snail (Lymnaea stagnalis) defence cells is regulated through integrated PKC, FAK and Src signalling.
Cell Tissue Res
; 341(1): 131-45, 2010 Jul.
Article
em En
| MEDLINE
| ID: mdl-20512591
ABSTRACT
Cell adhesion and spreading are vital to immune function. In molluscs, haemocytes (circulating phagocytes) are sentinels and effectors of the internal defence system; however, molecular mechanisms that regulate integrin-mediated spreading by haemocytes have not been characterised in detail. Visualisation of Lymnaea stagnalis haemocytes by scanning electron microscopy revealed membrane ruffling, formation of lamellipodia and extensive filopodia during early stages of cell adhesion and spreading. These events correlated with increased phosphorylation (activation) of protein kinase C (PKC) and focal adhesion kinase (FAK), sustained for 60 min. Treatment of haemocytes with the PKC inhibitors GF109203X or Gö 6976, or the Src/tyrosine kinase inhibitors SrcI or herbimycin A, attenuated haemocyte spread by 64, 46, 32 and 35%, respectively (P
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteína Quinase C
/
Transdução de Sinais
/
Movimento Celular
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Quinases da Família src
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Proteína-Tirosina Quinases de Adesão Focal
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Hemócitos
/
Lymnaea
Limite:
Animals
Idioma:
En
Revista:
Cell Tissue Res
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Reino Unido