Identification of four SUMO paralogs in the medaka fish, Oryzias latipes, and their classification into two subfamilies.
Biochem Genet
; 48(9-10): 737-50, 2010 Oct.
Article
em En
| MEDLINE
| ID: mdl-20549333
At least four paralogs of the small ubiquitin-related modifier (SUMO) exist in humans, but there is limited information about SUMO paralogs from other vertebrate species. We isolated the four cDNA encoding proteins, similar to human SUMOs, from the medaka fish, Oryzias latipes: OlSUMO-1, OlSUMO-2, OlSUMO-3, and OlSUMO-4. The amino acid sequences of OlSUMO-2, -3, and -4 are 89-94% identical, but they share only 45% identity with OlSUMO-1. Phylogenetic analysis, transient expression of OlSUMOs in cultured cells, and in vitro binding of OlSUMOs with two SUMO-interacting proteins demonstrated that the medaka SUMO paralogs can be grouped into two subfamilies, OlSUMO-1 and OlSUMO-2/3/4. Furthermore, this is the first report of all four OlSUMO transcripts being expressed in medaka embryos, implying that they have a role in fish development. This study will improve understanding of the relationship between structural and functional diversity of SUMO paralogs during vertebrate evolution.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oryzias
/
Homologia de Sequência de Aminoácidos
/
Proteína SUMO-1
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Biochem Genet
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Japão
País de publicação:
Estados Unidos