Odontoblast-like cytodifferentiation of human dental pulp cells in vitro in the presence of a calcium hydroxide-containing cement.
Arch Oral Biol
; 36(2): 117-28, 1991.
Article
em En
| MEDLINE
| ID: mdl-2059161
ABSTRACT
The cement produced microcrystals of calcite by reaction with culture medium supplemented with calf serum. Human dental pulp cells seeded on such a substrate preferentially adhered and aggregated around the microcrystals. Immunofluorescence and immunogold labelling revealed a high affinity of serum fibronectin molecules for the calcite crystals. At 4 weeks in culture, the cells had various features of differentiated odontoblasts, notably nuclear polarization, typical appearance of the Golgi apparatus, synthesis of type I collagen and absence of type III, and apical accumulation of actin and vimentin. These cells also elaborated a collagenous extracellular matrix which did not mineralize.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Hidróxido de Cálcio
/
Cimentos Dentários
/
Polpa Dentária
/
Odontoblastos
Limite:
Adolescent
/
Child
/
Humans
Idioma:
En
Revista:
Arch Oral Biol
Ano de publicação:
1991
Tipo de documento:
Article
País de afiliação:
França