[Effect of liposomal transfection of UCH-L1 siRNA on proliferation and apoptosis of lung cancer cell line H157].
Zhongguo Fei Ai Za Zhi
; 13(4): 292-6, 2010 Apr.
Article
em Zh
| MEDLINE
| ID: mdl-20677552
ABSTRACT
BACKGROUND AND OBJECTIVE:
Several reports demonstrated that the ubiquitin C terminal hydrolase-L1 (UCH-L1) has been found to be an oncogene in malignant tumors such as esophageal carcinoma, lung cancer and breast cancer. The aim of this study is to explore the effects of liposomal transfection of UCH-L1 siRNA on the proliferation and apoptosis of lung adenocarcinoma cell lines H157.METHODS:
UCH-L1 siRNA was synthesized and transfected into H157 cell by liposome. Cell morphological change was observed with microscope, and cell proliferation and apoptosis index detected by flow cytometry, UCH-L1 mRNA expression was determined by RT-PCR and protein level of UCH-L1 was determined by Western blot.RESULTS:
For the H157 cell transfected with siRNA, cell proliferation was inhibited significantly, cell apoptosis appeared obviously, the expression of UCH-L1 mRNA and protein level of UCH-L1 significantly decreased.CONCLUSION:
UCH-L1 siRNA is able to inhibit the proliferation of lung adenocarcinoma cell lines H157 and induce the apoptosis. UCH-L1 might become a new target for lung carcinoma gene therapy.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transfecção
/
Terapia Genética
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Apoptose
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RNA Interferente Pequeno
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Ubiquitina Tiolesterase
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Proliferação de Células
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Lipossomos
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Neoplasias Pulmonares
Limite:
Humans
Idioma:
Zh
Revista:
Zhongguo Fei Ai Za Zhi
Assunto da revista:
NEOPLASIAS
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
China
País de publicação:
CHINA
/
CN
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REPUBLIC OF CHINA