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Investigation of relaxant effects of new agents affecting nitric oxide/cyclic guanosine monophosphate pathway on sheep oddi sphincter.
Sarac, Bulent; Durmus, Nedim; Bagcivan, Ihsan; Altun, Ahmet; Turan, Mustafa; Sencan, Mehmet.
Afiliação
  • Sarac B; Departments of Pharmacology, Cumhuriyet University School of Medicine, Sivas, Turkey. saracbulent@mynet.com
Pancreas ; 39(6): 875-8, 2010 Aug.
Article em En | MEDLINE | ID: mdl-20697210
OBJECTIVES: Nitric oxide (NO) is a potent nonadrenergic, noncholinergic mediator of gastrointestinal smooth muscle. We aimed to investigate the effects of new NO/cyclic guanosine monophosphate (cGMP) pathway-affecting agents at the sheep sphincter of Oddi (SO) in vitro. METHODS: Sheep SO rings were mounted in organ baths and tested for isometric tension and cGMP levels in response to 3,3-bis(aminoethyl)-1-hydroxy-2-oxo-1-triazene; 3-morpholinosydnonimine hydrochloride (SIN-1); and BAY 41-2272 in the presence and absence of 1H-(1,2,4)oxadiazole(4,3-a)quinoxalin-1-one (ODQ). RESULTS: 3,3-bis(Aminoethyl)-1-hydroxy-2-oxo-1-triazene; SIN-1; and BAY 41-2272 relaxed SO rings in a concentration-dependent manner. These relaxations were significantly decreased in the presence of ODQ (P < 0.05). All agents significantly increased the cGMP levels compared with the control group (P < 0.05). The increased cGMP levels in the 3,3-bis(aminoethyl)-1-hydroxy-2-oxo-1-triazene- and BAY 41-2272-treated groups were significantly different from both control and carbachol groups (P < 0.05), whereas the increase in the SIN-1 group was significantly different from all groups (P < 0.05). The cGMP levels were significantly lower in the presence of ODQ compared with its absence (P < 0.05). CONCLUSIONS: The relaxation of SO rings by these agents may be via increasing the levels of cGMP. The additional increase produced by SIN-1 may be the combined effects of NO generation and activation of guanylyl cyclase.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oxidiazóis / Pirazóis / Piridinas / Quinoxalinas / Triazenos / Molsidomina / GMP Cíclico / Relaxamento Muscular / Músculo Liso / Óxido Nítrico Limite: Animals Idioma: En Revista: Pancreas Assunto da revista: GASTROENTEROLOGIA Ano de publicação: 2010 Tipo de documento: Article País de afiliação: Turquia País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oxidiazóis / Pirazóis / Piridinas / Quinoxalinas / Triazenos / Molsidomina / GMP Cíclico / Relaxamento Muscular / Músculo Liso / Óxido Nítrico Limite: Animals Idioma: En Revista: Pancreas Assunto da revista: GASTROENTEROLOGIA Ano de publicação: 2010 Tipo de documento: Article País de afiliação: Turquia País de publicação: Estados Unidos