Micellar electrokinetic chromatography method development for determination of impurities in ritonavir.
J Pharm Biomed Anal
; 53(5): 1210-6, 2010 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-20719446
ABSTRACT
Ritonavir is a synthetic peptidomimetic human immunodeficiency virus (HIV) protease inhibitor employed in the treatment of AIDS since 1996. Synthetic precursors are potential impurities in the final product. In the present work a micellar electrokinetic chromatography (MEKC) method for the separation of Ritonavir from three available synthetic precursors was developed. The optimized separation is performed in a background electrolyte composed of sodium tetraborate (pH 9.6; 15mM) containing sodium dodecylsulfate (30mM) and acetonitrile (18%, v/v). Mass spectrometry was used to confirm the identity of the tested substances. Good repeatability was observed for migration time (RSD about 0.4%) and peak area (RSD about 0.8%). The limits of detection (LOD) obtained allow the determination of two of the impurities at levels as low as 0.005% m/m, and one at a level of 0.3% m/m.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Contaminação de Medicamentos
/
Ritonavir
/
Cromatografia Capilar Eletrocinética Micelar
/
Micelas
Idioma:
En
Revista:
J Pharm Biomed Anal
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Bélgica