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Imaging mass spectrometry reveals unique lipid distribution in primary varicose veins.
Tanaka, H; Zaima, N; Yamamoto, N; Sagara, D; Suzuki, M; Nishiyama, M; Mano, Y; Sano, M; Hayasaka, T; Goto-Inoue, N; Sasaki, T; Konno, H; Unno, N; Setou, M.
Afiliação
  • Tanaka H; Division of Vascular Surgery, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu 431-3192, Japan.
Eur J Vasc Endovasc Surg ; 40(5): 657-63, 2010 Nov.
Article em En | MEDLINE | ID: mdl-20817502
ABSTRACT

BACKGROUND:

The lipid metabolism of varicose veins (VVs) remains unknown. To elucidate the pathogenesis of VV, we utilized the novel technique of imaging mass spectrometry (IMS). MATERIALS AND

METHODS:

We obtained VV tissues from 10 limbs of 10 VV patients who underwent great saphenous vein stripping. As control vein samples, we harvested segmental vein tissues from 6 limbs of 6 patients with peripheral artery occlusive disease who underwent infra-inguinal bypass with reversed saphenous vein grafting. To identify the localisation of lipid molecules in the VV tissues, we performed matrix-assisted laser desorption/ionization IMS (MALDI-IMS). We also performed MS/MS analyses to identify the structure of each molecule.

RESULTS:

We obtained mass spectra directly from control vein tissues and VV tissues and found a unique localisation of lipid molecules in the VV tissues. We localised lysophosphatidylcholine (LPC) (1-acyl 160), phosphatidylcholine (PC) (1-acyl 364) and sphingomyelin (SM) (d181/160) at the site of the VV valve.

CONCLUSION:

MALDI-IMS revealed the distribution of various lipid molecules in normal veins and VVs both. Accumulation of LPC (1-acyl 160), PC (1-acyl 364) and SM (d181/160) in the VV tissues suggested that inflammation associated with abnormal lipid metabolism may contribute to the development of VV.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Veia Safena / Varizes / Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz / Lipídeos Limite: Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Eur J Vasc Endovasc Surg Assunto da revista: ANGIOLOGIA Ano de publicação: 2010 Tipo de documento: Article País de afiliação: Japão

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Veia Safena / Varizes / Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz / Lipídeos Limite: Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Eur J Vasc Endovasc Surg Assunto da revista: ANGIOLOGIA Ano de publicação: 2010 Tipo de documento: Article País de afiliação: Japão