Imaging mass spectrometry reveals unique lipid distribution in primary varicose veins.
Eur J Vasc Endovasc Surg
; 40(5): 657-63, 2010 Nov.
Article
em En
| MEDLINE
| ID: mdl-20817502
ABSTRACT
BACKGROUND:
The lipid metabolism of varicose veins (VVs) remains unknown. To elucidate the pathogenesis of VV, we utilized the novel technique of imaging mass spectrometry (IMS). MATERIALS ANDMETHODS:
We obtained VV tissues from 10 limbs of 10 VV patients who underwent great saphenous vein stripping. As control vein samples, we harvested segmental vein tissues from 6 limbs of 6 patients with peripheral artery occlusive disease who underwent infra-inguinal bypass with reversed saphenous vein grafting. To identify the localisation of lipid molecules in the VV tissues, we performed matrix-assisted laser desorption/ionization IMS (MALDI-IMS). We also performed MS/MS analyses to identify the structure of each molecule.RESULTS:
We obtained mass spectra directly from control vein tissues and VV tissues and found a unique localisation of lipid molecules in the VV tissues. We localised lysophosphatidylcholine (LPC) (1-acyl 160), phosphatidylcholine (PC) (1-acyl 364) and sphingomyelin (SM) (d181/160) at the site of the VV valve.CONCLUSION:
MALDI-IMS revealed the distribution of various lipid molecules in normal veins and VVs both. Accumulation of LPC (1-acyl 160), PC (1-acyl 364) and SM (d181/160) in the VV tissues suggested that inflammation associated with abnormal lipid metabolism may contribute to the development of VV.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Veia Safena
/
Varizes
/
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
/
Lipídeos
Limite:
Aged
/
Aged80
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Eur J Vasc Endovasc Surg
Assunto da revista:
ANGIOLOGIA
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Japão