[Purification and properties of the hexon antigen of canine adenovirus serotype CAV-1]. / Ochistka i svoistva geksonobogo antigena adenovirusa sobak serotipa CAV-1.
Biokhimiia
; 55(11): 1996-2001, 1990 Nov.
Article
em Ru
| MEDLINE
| ID: mdl-2085617
ABSTRACT
The main antigen and immunogen of canine adenovirus type 1 (CAV-1) has been purified to near homogeneity from cultural fluid of a CAV-1-infected primary cell culture by hydrophobic and anion-exchange chromatography. The hexon native form (trimer) was shown to be resistant against denaturation by SDS under conditions of SDS-PAGE performed without heating the samples. The monomer chain of the CAV-1 hexon was apparently identical in terms of electrophoretic mobility with that of the previously sequenced BAV-3 hexon polypeptide (103 kDA). In blot enzyme immunoassay only native trimers of CAV-1 hexon were detected by cross-specific polyclonal and monoclonal anti-hexon antibodies.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Adenoviridae
/
Capsídeo
/
Proteínas do Capsídeo
/
Antígenos Virais
Limite:
Animals
Idioma:
Ru
Revista:
Biokhimiia
Ano de publicação:
1990
Tipo de documento:
Article