Platelet-rich plasma (PRP) impairs the craniofacial bone repair associated with its elevated TGF-ß levels and modulates the co-expression between collagen III and α-smooth muscle actin.
J Orthop Res
; 29(3): 457-63, 2011 Mar.
Article
em En
| MEDLINE
| ID: mdl-20922797
ABSTRACT
Transforming growth factor-ß (TGF-ß) is considered the main inducer of both the α-smooth muscle actin (α-SMA) phenotype and collagen synthesis and deposition and plays a significant role in the tissue repair and the development of fibrosis. Since the PRP constitutes an important source of TGF-ß and its efficacy on the craniofacial bone repair remains controversy, the aim of this study was to evaluate the effect of PRP in the presence of levels of TGF-ß on PRP samples, as well as in the presence of collagen III and α-SMA+ cells, while comparing these results by means of a histomorphometric analysis of the bone matrix and fibrous deposition on the bone repair. Four bone defects of 16 mm(2) were created on the calvarium of 21 rabbits. The surgical defects were treated with either particulate autograft, particulate autograft mixed with PRP and PRP alone. Animals were euthanized at 15, 30, and 45 days postoperative. Histomorphometric and immunohistochemical analyses were performed to assess repair time, as well as the expression of collagen III, and α-SMA. The histomorphometric results demonstrated intensive deposition of fibrous tissue while hinder bone deposition occurred in PRP groups. These results coincided with higher values of the TGF-ß on the PRP sample, also larger occurrence of diffuse collagen III deposition and higher presence of α-SMA+ cells spread among the fibrous tissue. Thus, the higher levels of TGF-ß associated with the both expression of collagen III and α-SMA on defect treated with PRP suggest that its biomaterial induce an effect that can be considered similarly to a fibroproliferative disorder.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Crânio
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Regeneração Óssea
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Fator de Crescimento Transformador beta
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Actinas
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Colágeno Tipo III
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Plasma Rico em Plaquetas
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
Idioma:
En
Revista:
J Orthop Res
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
EEUU
/
ESTADOS UNIDOS
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ESTADOS UNIDOS DA AMERICA
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EUA
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UNITED STATES
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UNITED STATES OF AMERICA
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US
/
USA