Spectrofluorometric determination of methocarbamol in pharmaceutical preparations and human plasma.
J Fluoresc
; 21(2): 555-61, 2011 Mar.
Article
em En
| MEDLINE
| ID: mdl-20936330
ABSTRACT
A simple, sensitive and rapid spectrofluorometric method for determination of methocarbamol in pharmaceutical formulations and spiked human plasma has been developed. The proposed method is based on the measurement of the native fluorescence of methocarbamol in methanol at 313 nm after excitation at 277 nm. The relative fluorescence intensity-concentration plot was rectilinear over the range of 0.05-2.0 µg/mL, with good correlation (r=0.9999), limit of detection of 0.007 µg/ mL and a lower limit of quantification of 0.022 µg/ mL. The described method was successfully applied for the determination of methocarbamol in its tablets without interference from co-formulated drugs, such as aspirin, diclofenac, paracetamol and ibuprofen, The results obtained were in good agreement with those obtained using the official method (USP 30).The high sensitivity of the method allowed the determination of the studied drug in spiked human plasma with average percentage recovery of 99.42 ± 3.84.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Fluorescência
/
Preparações Farmacêuticas
/
Metocarbamol
Limite:
Humans
Idioma:
En
Revista:
J Fluoresc
Assunto da revista:
BIOFISICA
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Egito