Dependency of γ-secretase complex activity on the structural integrity of the bilayer.
Biochem Biophys Res Commun
; 402(2): 291-6, 2010 Nov 12.
Article
em En
| MEDLINE
| ID: mdl-20937251
ABSTRACT
γ-secretase is a membrane protein complex associated with the production of Aß peptides that are pathogenic in Alzheimer's disease. We have characterized the activity of γ-secretase complexes under a variety of detergent solubilization and reconstitution conditions, and the structural state of proteoliposomes by electron microscopy. We found that γ-secretase activity is highly dependent on the physical state or integrity of the membrane bilayer--partial solubilization may increase activity while complete solubilization will abolish it. The activity of well-solubilized γ-secretase can be restored to near native levels when properly reconstituted into a lipid bilayer environment.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Membrana Celular
/
Secretases da Proteína Precursora do Amiloide
/
Bicamadas Lipídicas
Limite:
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Estados Unidos