A simple method to record parietal cells in the fundic mucosa in baboons.
In Vivo
; 24(5): 705-7, 2010.
Article
em En
| MEDLINE
| ID: mdl-20952737
ABSTRACT
BACKGROUND:
Gastric parietal cells in a baboon were recently found to be auto-fluorescent.AIM:
To study gastric sections with a fluorescent microscope in a cohort of baboons. MATERIAL ANDMETHODS:
Gastric sections from 38 baboons were stained with hematoxylin-eosin (H&E) and examined in a fluorescence microscope (FLM). The thickness of the parietal cell population was assessed at x 10 magnification.RESULTS:
H&E stained all mucosal cells fovelar, parietal and chief cells. When the same sections were analyzed with an FLM, only parietal cells were auto-fluorescent, whereas fovelar and chief cells remained non-fluorescent. Parietal cells formed a distinct, continuous auto-fluorescent band. The ratio of the auto-fluorescent parietal cell band/total mucosa ranged between 0.20 and 0.30.CONCLUSION:
Gastric parietal cells became auto-fluorescent when H&E-stained sections from baboon stomachs were observed with an FLM. Eosin was the stain responsible for this optical phenomenon.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células Parietais Gástricas
/
Amarelo de Eosina-(YS)
/
Corantes Fluorescentes
/
Fundo Gástrico
/
Microscopia de Fluorescência
Limite:
Animals
Idioma:
En
Revista:
In Vivo
Assunto da revista:
NEOPLASIAS
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Suécia