Towards a mouse model for sickle cell disease: HB SAD.
Nouv Rev Fr Hematol (1978)
; 32(6): 407-8, 1990.
Article
em En
| MEDLINE
| ID: mdl-2101873
Very recently a high expression of human hemoglobin S, which causes sickle cell disease, has been obtained in transgenic mice. We have constructed a modified beta S gene, beta SAD which carries two additional mutations in order to induce polymerization of transgenic hemoglobin when diluted by endogenous mouse Hb. The transgenic SAD mice are not anemic but exhibit a low percentage of irreversible sickle cells. Sickling is induced by deoxygenation of erythrocytes in vitro. In addition, the anemia of neonates and the low incidence of SAD animals in the progeny suggest a deleterious effect of SAD Hb during development. Finally, hypoxia induces a high mortality in SAD adults suggesting the induction of vaso-occlusive events.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Hemoglobina Falciforme
/
Camundongos Transgênicos
/
Anemia Falciforme
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
En
Revista:
Nouv Rev Fr Hematol (1978)
Ano de publicação:
1990
Tipo de documento:
Article
País de publicação:
Alemanha