Multifunctional CPP polymer system for tumor-targeted pDNA and siRNA delivery.
Methods Mol Biol
; 683: 453-63, 2011.
Article
em En
| MEDLINE
| ID: mdl-21053149
ABSTRACT
Cell-penetrating peptides (CPPs) are a very interesting class of molecules to be introduced in gene and siRNA vectors. They can be used to overcome one of the biggest hurdles in gene and siRNA delivery in vitro and in vivo, the transfer across cell membranes. This chapter describes protocols for the synthesis and biological evaluation of a polylysine-based polymer. In this carrier system, melittin is used as CPP with a high activity to disrupt membranes. pH-Labile masking is applied to render the lytic activity specific for intracellular acidic endolysosomal organelles.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polilisina
/
DNA
/
Transfecção
/
RNA Interferente Pequeno
/
Peptídeos Penetradores de Células
/
Neoplasias
Limite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Alemanha