An association of TLR216934A >T polymorphism and severity/phenotype of atopic dermatitis.
J Eur Acad Dermatol Venereol
; 25(6): 715-21, 2011 Jun.
Article
em En
| MEDLINE
| ID: mdl-21134221
ABSTRACT
BACKGROUND:
Toll-like receptor 2 gene (TLR2) 16934A>T polymorphism has been shown to be associated with severity of atopic dermatitis (AD) as measured using severity scoring of atopic dermatitis (SCORAD) index. Moreover, TLR216934A>T polymorphism has been associated with atopy and allergic disorders in farmers' children.OBJECTIVE:
The aim of this study was to evaluate an association between TLR216934A>T polymorphism and AD phenotype, including disease severity and concomitant atopic diseases, or potential serum markers of AD severity and also to find a molecular background of the clinical associations.METHODS:
Genotyping for TLR216934A>T polymorphism was performed in 130 consecutive adult ambulatory patients with AD. Total serum (TS) IgE levels, serum tryptase, plasma interleukin-6 and C-reactive protein were measured. In addition, luciferase assay and electrophoretic-mobility shift assay were conducted to assess the effect of 16934A>T polymorphism on transcriptional activity.RESULTS:
There was an inverse association of TLR216934TT genotype and/or 16934T allele with SCORAD, but not with TS IgE, tryptase or inflammatory markers. Interestingly, 16934AA genotype and/or 16934A allele were overrepresented in AD patients with concomitant asthma or a family history of atopy. In a subgroup analysis, TLR216934A>T polymorphism was associated with SCORAD, asthma, allergic conjunctivitis or family history of atopy in AD patients with TS IgE ≥106 IU/mL but not in those having TS IgE <106 IU/mL. Functional analyses showed that TLR216934T allele is associated with higher luciferase activity in human monocytic THP-1 cells and preferential binding of the THP-1-derived nuclear protein.CONCLUSION:
TLR216934A>T polymorphism could be a genetic predictor of AD severity, the coexistence of asthma or atopic conjunctivitis as well as a family history of atopic diseases, especially in subjects having higher TS IgE. TLR216934A>T polymorphism affects transcriptional activity, which may at least in part account for the clinical associations observed for the 16934A>T polymorphism.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polimorfismo Genético
/
Dermatite Atópica
/
Receptor 2 Toll-Like
Tipo de estudo:
Prognostic_studies
/
Risk_factors_studies
Limite:
Adult
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
J Eur Acad Dermatol Venereol
Assunto da revista:
DERMATOLOGIA
/
DOENCAS SEXUALMENTE TRANSMISSIVEIS
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Japão