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High claudin-7 expression is associated with a poor response to platinum-based chemotherapy in epithelial ovarian carcinoma.
Kim, Chul Jung; Lee, Jeong-Won; Choi, Jung-Joo; Choi, Hye Young; Park, Young-Ae; Jeon, Hye-Kyung; Sung, Chang Ohk; Song, Sang Yong; Lee, Yoo-Young; Choi, Chel Hun; Kim, Tae-Joong; Lee, Je-Ho; Kim, Byoung-Gie; Bae, Duk-Soo.
Afiliação
  • Kim CJ; Department of Obstetrics and Gynecology, College of Medicine, Konyang University, Daejeon 302-718, Republic of Korea.
Eur J Cancer ; 47(6): 918-25, 2011 Apr.
Article em En | MEDLINE | ID: mdl-21134740
BACKGROUND: Claudin-7 (CLDN-7) is a tight junction protein that has been shown overexpressed in several human cancers. We investigated prognostic significance of CLDN-7 overexpression in patients with epithelial ovarian carcinoma (EOC) and its functional role on cell proliferation in ovarian carcinoma cell lines. PATIENTS AND METHODS: CLDN-7 expression was evaluated by real-time RT-PCR and immunohistochemical analysis in 71 patients with EOC. We assessed the association of CLDN-7 expressions with prognosis of the patients including sensitivity to platinum-based chemotherapy. In vitro experiment was performed with and without inhibition of CLDN-7 by its siRNA to evaluate the sensitivity of the human ovarian cancer cells to cisplatin chemotherapy. RESULTS: CLDN-7 transcripts in EOCs were significantly up-regulated compared with normal ovarian tissues (P<0.001). The expression of CLDN-7 protein was observed in majority (69/71, 97.1%) of the EOCs but not in normal ovarian tissues (P<0.001). High CLDN-7 expression in primary tumour correlated with shorter progression-free survival (PFS) of the patients (P=0.005) and poor sensitivity to platinum-based chemotherapy (P=0.024). Moreover, CLDN-7 was highly expressed in 2774 and HeyA8 human ovarian cancer cells and inhibition of CLDN-7 by its siRNA significantly enhanced the sensitivity of 2774 and HeyA8 cells to cisplatin treatment. CONCLUSION: These findings suggest CLDN-7 expression is an independent prognostic factor for PFS and it may play a role in regulating response to platinum-based chemotherapy in the treatment of EOC.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Ovarianas / Cisplatino / Neoplasias Epiteliais e Glandulares / Proteínas de Membrana / Antineoplásicos Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Adult / Aged / Female / Humans / Middle aged Idioma: En Revista: Eur J Cancer Ano de publicação: 2011 Tipo de documento: Article País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Ovarianas / Cisplatino / Neoplasias Epiteliais e Glandulares / Proteínas de Membrana / Antineoplásicos Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Adult / Aged / Female / Humans / Middle aged Idioma: En Revista: Eur J Cancer Ano de publicação: 2011 Tipo de documento: Article País de publicação: Reino Unido