Your browser doesn't support javascript.
loading
Poly(T) variation in heteroderid nematode mitochondrial genomes is predominantly an artefact of amplification.
Riepsamen, Angelique H; Gibson, Tracey; Rowe, Janet; Chitwood, David J; Subbotin, Sergei A; Dowton, Mark.
Afiliação
  • Riepsamen AH; School of Biological Sciences, University of Wollongong, Wollongong, NSW 2522, Australia. ahr96@uow.edu.au
J Mol Evol ; 72(2): 182-92, 2011 Feb.
Article em En | MEDLINE | ID: mdl-21161202
ABSTRACT
We assessed the rate of in vitro polymerase errors at polythymidine [poly(T)] tracts in the mitochondrial DNA (mtDNA) of a heteroderid nematode (Heterodera cajani). The mtDNA of these nematodes contain unusually high numbers of poly(T) tracts, and have previously been suggested to contain biological poly(T) length variation. However, using a cloned molecule, we observed that poly(T) variation was generated in vitro at regions containing more than six consecutive Ts. This artefactual error rate was estimated at 7.3 × 10(-5) indels/poly(T) tract >6 Ts/cycle. This rate was then compared to the rate of poly(T) variation detected after the amplification of a biological sample, in order to estimate the 'biological + artefactual' rate of poly(T) variation. There was no significant difference between the artefactual and the artefactual + biological rates, suggesting that the majority of poly(T) variation in the biological sample was artefactual. We then examined the generation of poly(T) variation in a range of templates with tracts up to 16 Ts long, utilizing a range of Heteroderidae species. We observed that T deletions occurred five times more frequently than insertions, and a trend towards increasing error rates with increasing poly(T) tract length. These findings have significant implications for studies involving genomes with many homopolymer tracts.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poli T / Tylenchoidea / Variação Genética / Artefatos / Técnicas de Amplificação de Ácido Nucleico / Genoma Helmíntico / Genoma Mitocondrial Limite: Animals Idioma: En Revista: J Mol Evol Ano de publicação: 2011 Tipo de documento: Article País de afiliação: Austrália

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poli T / Tylenchoidea / Variação Genética / Artefatos / Técnicas de Amplificação de Ácido Nucleico / Genoma Helmíntico / Genoma Mitocondrial Limite: Animals Idioma: En Revista: J Mol Evol Ano de publicação: 2011 Tipo de documento: Article País de afiliação: Austrália