Interaction between Core protein of classical swine fever virus with cellular IQGAP1 protein appears essential for virulence in swine.
Virology
; 412(1): 68-74, 2011 Mar 30.
Article
em En
| MEDLINE
| ID: mdl-21262517
ABSTRACT
Here we show that IQGAP1, a cellular protein that plays a pivotal role as a regulator of the cytoskeleton interacts with Classical Swine Fever Virus (CSFV) Core protein. Sequence analyses identified residues within CSFV Core protein (designated as areas I, II, III and IV) that maintain homology to regions within the matrix protein of Moloney Murine Leukemia Virus (MMLV) that mediate binding to IQGAP1 [EMBO J, 2006 252155]. Alanine-substitution within Core regions I, II, III and IV identified residues that specifically mediate the Core-IQGAP1 interaction. Recombinant CSFV viruses harboring alanine substitutions at residues (207)ATI(209) (I), (210)VVE(212) (II), (213)GVK(215) (III), or (232)GLYHN(236) (IV) have defective growth in primary swine macrophage cultures. In vivo, substitutions of residues in areas I and III yielded viruses that were completely attenuated in swine. These data shows that the interaction of Core with an integral component of cytoskeletal regulation plays a role in the CSFV cycle.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas do Core Viral
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Proteínas Ativadoras de ras GTPase
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Mapeamento de Interação de Proteínas
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Interações Hospedeiro-Patógeno
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Vírus da Febre Suína Clássica
Limite:
Animals
Idioma:
En
Revista:
Virology
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Estados Unidos