[Effect of sodium diphenylhydantoin on sister chromatid exchange frequency in mice]. / Efecto del difenilhidantoinato de sodio (DFH) sobre la frecuencia de intercambio de cromátides hermanas (ICH) en ratones.

ABSTRACT

In order to analyze the effect of the anticonvulsant diphenyl hydantoin (DPH) over the frequency of sister chromatid exchanges (SCE) in vivo, Balb-C mouse 8-12 weeks old, males and females, were injected intraperitoneally with a single dose and three different concentrations 1, 10 and 20 mg/Kg, as negative and positive control, we used physiological serum and mitomycin-C respectively that were injected in the same way. Twenty five metaphases were analyzed per individual in the slide with the differential staining of sister chromatid, we found a light, but significantly increase, in the frequency of SCE in the concentrations of 10 and 20 mg/Kg when they were compared with the ones obtained for the negative control. In the males was observed a positive correlation in the levels of ICH when the doses of DPH were increased.