The chitinolytic activities of Streptomyces sp. TH-11.
Int J Mol Sci
; 12(1): 56-65, 2010 Dec 27.
Article
em En
| MEDLINE
| ID: mdl-21339977
ABSTRACT
Chitin is an abundant biopolymer composed of units of N-acetyl-D-glucosamine linked by ß-1,4 glycosidic bonds. Chitin is the main component of the shells of mollusks, the cell wall of fungi and yeast and of the exoskeleton of crustaceans and insects. The degradation of chitin is catalyzed by chitinases that occur in a wide range of organisms. Among them, the chitinases from microorganisms are extremely important for the degradation and recycling of the carbon and nitrogen trapped in the large amount of insoluble chitin in nature. Streptomyces sp. TH-11 was isolated from the sediment of the Tou-Chien River, Taiwan. The chitinolytic enzyme activities were detected using a rapid in-gel detection method from the cell-free preparation of the culture medium of TH-11. The chitinolytic enzyme activity during prolonged liquid culturing was also analyzed by direct measurement of the chitin consumption. Decomposition of the exoskeleton of shrimps was demonstrated using electron microscopy and atomic force microscopy.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Streptomyces
/
Proteínas de Bactérias
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Quitina
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Quitinases
Limite:
Animals
Idioma:
En
Revista:
Int J Mol Sci
Ano de publicação:
2010
Tipo de documento:
Article