Molecular typing and antimicrobial resistance of Salmonella Enteritidis isolated from poultry, food, and humans in Serbia.
Folia Microbiol (Praha)
; 56(1): 66-71, 2011 Jan.
Article
em En
| MEDLINE
| ID: mdl-21394475
Molecular typing and resistotyping coupled with gyrA single nucleotide polymorphism (SNP) of 60 Salmonella Enteritidis (SE) isolates originated from poultry, food, and humans in Serbia is described. Molecular fingerprinting was performed by randomly amplified polymorphic DNA (RAPD) using four primers, and the diversity index (D) was 0.688. In combination with resistotyping and gyrA SNP, D increased to 0.828. A total of 23 genetic groups were obtained. When four RAPD primers were combined, epidemic isolates from a fast-food restaurant outbreak were clustered in a distinctive genetic group. Among 60 SE strains, three had multiple resistances to three or more antibiotics. Nine strains were resistant to nalidixic acid (NAL; a non-fluorinated quinolone). The mutations in quinolone resistance-determining region (QRDR) found in NAL-resistant strains were attributed to Asp(87) â Asn in six strains, Asp(87) â Gly in one strain, and Ser(83) â Phe in one strain. One NAL-resistant strain had no mutations in QRDR, suggesting another mechanism of resistance.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doenças das Aves Domésticas
/
Salmonella enteritidis
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Infecções por Salmonella
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Salmonelose Animal
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Farmacorresistência Bacteriana
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Microbiologia de Alimentos
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Antibacterianos
Limite:
Animals
/
Humans
País/Região como assunto:
Europa
Idioma:
En
Revista:
Folia Microbiol (Praha)
Ano de publicação:
2011
Tipo de documento:
Article
País de publicação:
Estados Unidos